Farsamaynta Jiilka Xiga ee Samaynta Mouse-ka Mouse
TurboMice™ Technology
Waxaa soo saaray MingCeler iyada oo loo marayo taxanayaal hagaajin ah oo dhammaystirka tetraploid.
Marka la isku daroFarsamada Tafatirka Hid ee saxda ahiyo jiirka la hagaajiyayFarsamada Diyaarinta Unugyada Jirka Embryonic, waxaan hadda wax ka beddeli karnaa ku dhawaad goob kasta oo hidde-sidaha la beegsanayo.
TurboMice™ Technology
Tiknooloojiyada dhameystirka Tetraploid ee waxtarka leh ayaa noo ogolaatay inaan si aad ah u kordhino heerka dhalashada jiirarka laga bilaabo 1%-5% ilaa 30%-60% oo leh unugyada tarma ee hiddo-sidaha la tafatiray, taasoo ku dhow inay la mid noqoto waxtarka wareejinta embriyaha caadiga ah.
MingCeler waa shirkaddii ugu horreysay ee adduunka si guul leh u fulisa isbeddelka Tetraploid Complementation technology ka shaybaarka una gudubta codsiga warshadaha.
Farsamada Dhaqanka
Tiknoolajiyada qaabaynta xayawaanka dhaqameed, sida microinjection pronuclear iyo ES bartilmaameedka, waxay ku lug leedahay taranka ugu yaraan 2 ilaa 3 jiil si loo helo jiirarka genotype-ka qanjirka ah, kaas oo caadi ahaan qaata 6-8 bilood.
Nidaamkan dheer wuxuu si weyn u carqaladeeyaa horumarka iyo waxtarka horumarinta daroogada cusub iyo cilmi-baarista cudurrada.
Dufcada Degdega ah
1. ilaa 20 jiir hiddo-sidaha qaniisyada ah oo gudaha ku jira2-4 bilood.
2. ≈ 50 jiir qaniis ah oo ku dhex jira kuwan soo socda2 bilood
3. 400+ jiir qaniis ah oo ku dhex jira8-12 bilood
Warbixinta Kiis 2020
Tignoolajiyada TurboMice™ waxaa loo adeegsaday in si degdeg ah loo soo saaro500 oo homozygous ahJiirarka ACE2 ee bani'aadamnimada lagu sameeyay gudaha kaliya8 biloodloogu talagalay daraasaadka daawada COVID-19 iyo tallaalka.
Xulashada Culayska Dabacsan
Farsamooyinka dhaqameedku waxay leeyihiin xaddidaadyo la taaban karo marka la dooranayo culeyska jiirka, halka tignoolajiyada TurboMice™ ay bixiso dabacsanaan badan oo leh noocyo kala duwan oo la jeexjeexay iyo kuwa ka soo baxay oo laga dooran karo (oo ay ku jiraan Balb / c, ICR, C57BL/6, iwm.).
● Jiirarka jiilka F0 ee lagu dhisay tignoolajiyada TurboMice™ waa jiirarka la beegsanayo ee asalkoodu yahay hal unug.
● Sidaa darteed, walxaha hiddaha ee jiirarka F0 waa isku mid, taasoo yaraynaysa khaladaadka tijaabada ah ee ay sababaan kala duwanaanshaha walxaha hidde-sideyaasha.
● Sidaas awgeed, xogta tijaabada ah ee qiimaynta waxtarka daroogada iyo tijaabooyinka kale ayaa ah kuwo joogto ah oo la isku halayn karo.
Waxay ilaalisaa daacadnimada Hiddaha Wanaagsan
Gudaha Situ Precision Gene Editing
● Meesha tafatirka saxda ah ee hidde-sidaha oo leh heerar sax ah oo muujinta hidde-sidaha iyo gaarnimada unugyada saxda ah.
● Jiirarka ACE2 ee bani'aadamnimada lagu sameeyay iyadoo loo marayo farsamooyin dhaqameed ayaa waxaa soo saaray hordhaca dibadda ee K18-ACE2, kaas oo aan awoodin inuu gaaro tafatirka saxda ah sababtoo ah gelinta aan tooska ahayn, taasoo keentay la'aanta unugyada gaarka ah ee muujinta ACE2 ee qaabka bini'aadamka ee la dhisay ACE2 mouse model .
● Jiirarka ACE2 ee bani-aadminimo ee MingCeler waxay soo bandhigaan odhaah gaar ah oo ka mid ah xubnaha kala duwan (garaafyada C iyo D ee kor ku xusan), waxayna si wax ku ool ah u ekaysiiyaan sifooyinka bukaan-socodka kadib caabuqa SARS-CoV-2.
Horumarinta Gaarka ah ee Platform
●MingCeler madal lahaanshaha EnhancerPlus waxay ka caawin kartaa macaamiisheena inay horumariyaan heerarka muujinta hiddo-wadaha aadanaha.
● Natiijadii sannadaha ururinta xogta ee epigenetics iyo cilmi-baarista go'aaminta masiirka unugga, MingCeler's EnhancerPlus platform waxay si sax ah u saadaalin kartaa boosaska genomic ee Kobciyeyaasha, samaynta naqshadaynta xeeladaha tafatirka hidda-socodka caddayn ku salaysan.
● Natiijo ahaan, hiddo-wadeyaasha la beegsanayo waxay si ka wanaagsan ugu ekaan karaan qaababka muujinta iyo heerarka hiddo-wadaha endogenous.
Warbixinta kiiska: Bani'aadamnimada hidda-wadaha X.
● Ka faa'iidaysiga goobta 'EnhancerPlus platform', istaraatijiyadu waa la wanaajiyey, heerka hadalkuna wuxuu arkay koror saddex amar ah oo cabbir ah (fiiri garaafka kore), oo gaadhay shuruudaha horumarinta daroogada sida uu go'aamiyo macaamilka heerka borotiinka.
Ka dib markii la dhammeeyey wax-soo-saarka guud ee adduunka ugu horreeya ee ACE2 jiirarka bani'aadamnimada ee 2020, MingCeler waxay cusboonaysiisay ACE2 qaabka jiirka bani-aadamka iyada oo loo marayo afar isku-laabasho iyada oo loo marayo kor u qaadida EnhancerPlus, iyada oo heerka muujinta ACE2 bani'aadamnimo ugu dambeyntii gaadhay meel u dhow muujinta jiirka ACE2.
Heerarka muujinta ACE2 ee bani-aadmiga ee xubnaha kala duwan ee moodooyinka jiirka ee bani-aadmiga
Multi-Locus Hida Tafatirka
● Tiknoolajiyada TurboMice™ waa doorashada ugu fiican suuqa ee soo saarista moodooyinka jiirka ee isku dhafan ee isku dhafan ee meelo badan leh, taas oo siinaya faa'iidooyinka saxnaanta sare ee gelinta goobta, gelinta jajabka dheer, iyo meelo badan oo hidde ahaan wax laga beddelay, oo aan lahayn arrimo kala soocid.
Tiknoolajiyada TurboMice™ waxay u oggolaaneysaa in si isku mar ah loo tafatiro illaa 3 hiddo-sideyaal si loo soo saaro moodooyinka jiirka ee hal-abuurka leh ee isku-dhafan ee si toos ah uga imanaya unugyada tarma ee uurjiifka tafatiray, iyada oo aan loo baahnayn hababka taranka/baadhista waqti-qaadka ah, taasoo bixisa noocyo kala duwan. ee moodooyinka qalafsan ee qiimaha sare leh ee looga baahan yahay cilmi-baarista daroogada cusub.
● MingCeler waxa ay si guul leh u soo saartay noocyo kala duwan oo loci hiddo-wade-ku-habboon muddo gaaban gudaheed, oo ku salaysan ACE2 jiirarka bani-aadmiga.
Tafatirka Hiddaha Jajabka Dheer
Tiknoolajiyada TurboMice ™ waxay awood u siineysaa tafatirka saxda ah ee hidda-wadaha jajabyada dhaadheer ee ka badan 20kb, fududeynta wax soo saarka degdega ah ee moodooyinka kakan sida bini'aadantinimada, garaaca shuruuda ah (CKO), iyo garaaca jajabka weyn (KI).
Daabacaad
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin waxay xidhaa ACE2 si ay u xannibto isdhexgalka ay la leedahay SARS-CoV-2 borotiinka spike waxayna wax ku ool u tahay joojinta caabuqa SARS-CoV-2 ee moodooyinka xayawaanka.Cell Res.2021 Jan; 31 (1): 17-24.doi: 10.1038/s41422-020-00450-0.( HADDII: 20.507 )
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR Chen X, Pei D, Wu G, Zheng YT.Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwa285.( HADDII: 16.693 )
Socodka Adeegga
Waxa kaliya ee aad u baahan tahay inaad sameyso waa inaad na siisaa shuruudahaaga beddelka hidda-wadaha ee moodeelkaaga jiirka.Iyada oo ku saleysan macluumaadkaaga, waxaan isku dubaridi doonaa qorshe horudhac ah oo la habeeyey, ka dib dood dheeraad ah iyo heshiis labada dhinac ah, waxaan saxiixi doonaa qandaraas adeeg farsamo.Ka dib bilawga mashruuca waxaan ku siin doonaa warar cusub oo ku saabsan horumarka.