Khadadka unugga hidda-sidaha oo tafatiray
Khadadka unugyada hidda-wadaha-lagu tafatiray waxay tixraacaan unugyada lagu sameeyay isbeddellada hidde-sidaha iyada oo loo marayo habka loo yaqaan tafatirka hidda-socodka.Tafatirka hidda-wadaha waxa ay ku lug leedahay isbeddel lagu sameeyo DNA-da noolaha, inta badan iyada oo ujeedadu tahay in la barto hiddo-wadaha gaarka ah ama la fahmo hababka noolaha qaarkood.
Isticmaalka tignoolajiyada tafatirka hidda-wadaha, waxaa suurtagal ah in la gaaro khadadka unugyada unugyada firfircoonida, xadhkaha unugyada garaaca, isbeddelka dhibcaha, ama khadadka unugyada unugyada unugyada gaarka ah, kuwaas oo markaa loo isticmaali karo in lagu barto shaqada hidda-wadaha, calaamadaha, hababka cudurka , iyo horumarinta daroogada, iyo sidoo kale in lagu calaamadiyo unugyo gaar ah.
Waxaa jira dhowr sababood oo ah sababta khadadka unug ee hiddo-sidaha loo tafatiray ay qiimo ugu leeyihiin cilmi-baarista sayniska.Marka hore, waxay bixiyaan qalab lagu barto shaqada hiddo-wadaha gaarka ah.Beddelidda hidda-sidaha ku jira deegaan la kantaroolo, saynisyahannadu waxay u eegi karaan saameynta isbeddelka hidda-wadaha ee hab-dhaqanka unugyada ama hababka kale ee noolaha.Aqoontaani waxay gacan ka geysan kartaa faham wanaagsan oo ku saabsan cudurrada hidde-sideyaasha, iyo sidoo kale inay aqoonsato bartilmaameedyada daweynta ee suurtagalka ah.
Intaa waxaa dheer, khadadka unugyada hiddo-wade-ku-beddelka ayaa loo isticmaali karaa in lagu sameeyo oo lagu tijaabiyo dawooyin cusub.Soo bandhigida isbeddellada hidde-sidaha gaarka ah, saynisyahannadu waxay abuuri karaan khadadka unugyada kuwaas oo u ekaysiiya xaaladaha cudurrada, iyaga oo awood u siinaya inay qiimeeyaan waxtarka daaweynta suurtagalka ah.Tani waxay u oggolaaneysaa hababka horumarinta daroogada si sax ah oo hufan.
Waxaa intaa dheer, khadadka unugyada hiddo-wadaha-tafatirka ayaa leh suurtagalnimada codsiyada daawada dib-u-cusboonaynta.Iyadoo wax laga beddelayo hiddo-wadaha ka mas'uulka ah hab-dhaqanka unugyada ama kala-duwanaanta, saynis-yahannadu waxay kor u qaadi karaan awoodda daaweynta ee unugyada asliga ah ama waxay abuuri karaan unugyo ku habboon tallaalka.
Faa'iidada
1. Meelo badan oo hiddo-wadaha isku mar tafatiray;
2. Bixinta adeeg hal-joojin ah laga bilaabo naqshadaynta iyo isku-dhafka sgRNA ilaa baadhista khadka gacanta;
3. Khibrad qani ah oo ku saabsan soo saarista hiddo-wadaha si ay uga caawiso tafatirka hidde-sideyaasha laga bilaabo naqshadaynta nidaamka ilaa dhamaystirka dhismaha.
Ganacsiga ugu weyn
- Khadadka Unugyada Jidka-garaaca
- Khadadka unugyada firfircoonida hidda-wadaha
- Tilmaan xadhkaha unugyada mutant
- Khadadka Unugyada ee lagu garaaco
Tixraacyo
[1]Zhang S, Shen J, Li D, Cheng Y. Xeeladaha gudbinta Cas9ribonucleoprotein ee CRISPR/Cas9 genome editing.Dawaynta.2021 Jan1;11 (2):614-648.doi: 10.7150/thno.47007.PMID: 33391496;PMCID: PMC7738854.